Confinement plus Myosin-II suppression maximizes heritable loss of chromosomes, as revealed by live-cell ChReporters
The mechanical environment of a cell can have many effects, but whether it impacts the DNA sequence of a cell has remained unexamined. To investigate this, we developed a live-cell method to measure changes in chromosome numbers. We edited constitutive genes with GFP or RFP tags on single alleles and discovered that cells that lose … Read more